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GrainGenes Marker Report: WMC69

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Locus	wmc69
Type	Microsatellite
Map	Physical-3B 63.63
	[ Show Nearby Loci ]
Map Data	3B Physical
Probe	WMC69
Possible Orthologs	Xwmc69.1 Xwmc69.2
Probe	WMC69
Locus	Xwmc69.2 Xwmc69.1 wmc69
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1b12 Donor: Waugh, SCRI (CT)13 352 to 377. (2-3 polymorphic loci?).
Type	PCR SSR
PCR primers	Forward TcTgcTcTTAgcTAggAcgATg at 291 length 22 bases, Tm 61.0 Reverse cAgATgTTccccTTcAAAATgg at 500 length 22 bases, Tm 64.0
SSR size	231 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC69
Source Species	Triticum aestivum