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GrainGenes Marker Report: WMC70

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Probe	WMC70
Locus	Xwmc70 Xwmc70-7B wmc70
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1d12 Donor: Waugh, SCRI Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 220 bases located on 7B (CA)22 96 to 139, (CA)24 141 to 188. (1 polymorphic locus). Close relative (duplicate?) of 1d2, 1G4 - do they detect at least 1 common locus?
Type	PCR SSR
PCR primers	Forward ggggAgcAcccTcTATTgTcTA at 22 length 22 bases, Tm 63.0 Reverse TAATgcTcccAggAgAgAgTcg at 213 length 22 bases, Tm 65.0
SSR size	213 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC70
Source Species	Triticum aestivum