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GrainGenes Marker Report: WMC79

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Probe	WMC79
Locus	Xwmc079-4A [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6h7 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 132 bases located on 4D, 150 bases located on 4A AGR104. Trimmed to insert. Complex microsatellite (TC)(CA)104-164. 5' side of microsatellite has a match to 2e10 etc.
Type	PCR SSR
PCR primers	Forward cATcAATgcATATggcTgAAAT at 55 length 22 bases, Tm 61 Reverse AAAAgTTgTcATgAgcgAAgAA at 185 length 22 bases, Tm 61
SSR size	152 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC79
Source Species	Triticum aestivum