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GrainGenes Marker Report: WMC8

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Probe	WMC8
Locus	Xwmc8 wmc8 wmc8-4B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1A4 Donor: Nickerson Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 105 bases located on 4B AGR25. CA microsatellite, one main and several small, few repeats also using GCAC theme. Trimmed to Rsa1 fragment.
Type	PCR SSR
PCR primers	Forward cAcgcgcAcATcTcgccAAcTAA at 115 length 23 bases, Tm 73 Reverse cgTggTcTAgTccgcgTTgggTc at 214 length 23 bases, Tm 72
SSR size	122 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide;
Sequence	WMC8
Source Species	Triticum aestivum