Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: WMC85

[Submit comment/correction]

Locus	wmc85
Type	Microsatellite
Map	Durum Wheat, NSGC Lr 3B 66.3 Physical-3B 60.42
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr
Probe	WMC85
Possible Orthologs	Xwmc85 [ Show all 13 ]
Probe	WMC85
Locus	Xwmc85-1B [ Show all 14 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6g9 Donor: Devaux, Florimond Desprez AGR119. Trimmed to insert at 3' end(CA)8 153 to 168, (CA)18 184 to 219. Products a little weak at 61C, try lower anneal?
Type	PCR SSR
PCR primers	Forward ggAgTAAgAgAAAcATgccgAA at 27 length 22 bases, Tm 62 Reverse gTgcATgcATgAgAATAggAAc at 233 length 22 bases, Tm 61
SSR size	228 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC85
Source Species	Triticum aestivum