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GrainGenes Marker Report: WMC89

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Probe	WMC89
Locus	Xwmc89-4B [ Show all 18 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6a12 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 125 bases located on 4A, 140 bases located on 4D, 178 bases located on 4B AGR137. Trimmed to insert. (CA)19 64 to 101, (CT)8 40 to 55. Several polymorphic bands. Contain sequence element that matches 6C4 (complement of last 68 bases match bases 104-172 of 6C4) ie' 3' side of microsatellite.
Type	PCR SSR
PCR primers	Forward ATgTccAcgTgcTAgggAggTA at 6 length 22 bases, Tm 65 Reverse TTgccTcccAAgAcgAAATAAc at 160 length 22 bases, Tm 64
SSR size	176 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC89
Source Species	Triticum aestivum