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GrainGenes Marker Report: WMC94

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Probe	WMC94
Locus	Xwmc94-7D Xwmc94
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7d1 Donor: Somers, Agriculture Canada Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 109 bases located on 7D Multiple inserts (2) in this clone, joined 299-316 by linkers. Trimmed to insert at both ends. (GT)19 377 to 414 in second insert.
Type	PCR SSR
PCR primers	Forward TTcTAAAATgTTTgAAAcgcTc at 339 length 22 bases, Tm 58 Reverse gcATTTcgATATgTTgAAgTAA at 424 length 22 bases, Tm 56
SSR size	107 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC94
Source Species	Triticum aestivum