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GrainGenes Marker Report: WMC96

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Probe	WMC96
Locus	Xwmc96-4A [ Show all 16 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7d2 Donor: Somers, Agriculture Canada Trimmed to insert at both ends. (CA)29 177 to 234. (1 locus polymophic out of 3)
Type	PCR SSR
PCR primers	Forward TAgcAgccATgcTTAgcATcAA at 28 length 22 bases, Tm 65 Reverse gTTTcAgTcTTTcAcgAAcAcg at 286 length 22 bases, Tm 62
SSR size	280 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC96
Source Species	Triticum aestivum