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GrainGenes Marker Report: WMS182

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Probe
WMS182
Locus
Xgwm182-5D
Xgwm182-6B
Xgwm182
gwm182
gwm182-5D
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Remarks
Insert contains repeat (CT)18
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' TGATGTAGTGAGCCCATAGGC 3'
5' TTGCACACAGCCAAATAAGG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS182
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring