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GrainGenes Marker Report: WMS257

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Probe
WMS257
Locus
Xgwm257-2B
Xgwm257a
Xgwm257b
gwm257
Xgwm257
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Remarks
Insert contains repeat (GT)30
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' AGAGTGCATGGTGGGACG 3'
5' CCAAGACGATGCTGAAGTCA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS257
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring