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GrainGenes Marker Report: XHVM40g

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Locus	XHVM40g
Type	Microsatellite
Chromosome	5R
Chromosome Arm	5RS
Map	Secale-UxE-5 49.9
	[ Show Nearby Loci ]
Map Data	Rye, UC90 x E-line
Probe	HVM40
Probe for Locus	HVM40
Locus	HVM40 [ Show all 6 ]
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
General Remarks	Insert contains repeats (GA)6, (GT)4, and (GA)7.
Type	PCR SSR
PCR primers	5' CGATTCCCCTTTTCCCAC 3' 5' ATTCTCCGCCGTCCACTC 3' CGATTCCCCTTTTCCCAC ATTCTCCGCCGTCCACTC
SSR size	0.160
Amplification Conditions	The 'touchdown' PCR comprised 48 cycles of 94oC for 1 min denaturing and 72oC for 1 min extension. Annealing (30 s) temperatures were progressively decreased by 1oC every second cycle from 64oC to 55oC. Annealing conditions of 1 min at 55oC were maintained during the final 30 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.