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GrainGenes Marker Report: Xbarc100

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Locus	Xbarc100
Type	Microsatellite
Chromosome	5A
Map	Ta-NxW-5A 78.4 Wheat, WH542 x Synthetic 5A 65.3 Wheat-Composite2004-5A 28
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Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, WH542 x Synthetic
Probe	BARC100
Possible Orthologs	Xbarc100-2A Xbarc100-5A barc100 barc100-5A
Probe for Locus	BARC100
Locus	Xbarc100-2A Xbarc100-5A Xbarc100 barc100 barc100-5A
General Remarks	Contains motif : (TAA)19 BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type	PCR SSR
PCR primers	5' CCGTTAAGCCGCCTACCACAGAGTTGC 3' 5' GGCTTAAAACGGATCCTCCAGGTCAT 3'
Amplification Conditions	Annealing temperature = 52
Sequence	BV211582
Source Species	Triticum aestivum