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GrainGenes Marker Report: Xbarc114

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Locus
Xbarc114
Type
Microsatellite
Map
Wheat, UC1110 x PI610750 4B76.4
[ Show Nearby Loci ]
Map Data
Wheat, Synthetic x Opata, BARC
Wheat, UC1110 x PI610750
Probe
BARC114
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs
Xbarc114-2B
barc114



Probe for Locus
BARC114
Locus
Xbarc114
Xbarc114-2B
barc114
General Remarks
Contains motif : (ATT)17
SSR size is the band size in Chinese Spring
BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type
PCR
SSR
PCR primers
5' GGG TAT CAC GCA TTG TAA AAT CCG AAA 3'
5' GCT GAC TTC CGC AAA TGC CAT TCG CTG AT 3'
SSR size
147
Amplification Conditions
Annealing temperature = 50
Sequence
BV211507
Source Species
Triticum aestivum