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GrainGenes Marker Report: Xbarc132

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Locus
Xbarc132
Type
Microsatellite
Map
Wheat, UC1110 x PI610750 3D25.2
[ Show Nearby Loci ]
Map Data
Wheat, Synthetic x Opata, BARC
Wheat, UC1110 x PI610750
Probe
BARC132
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs
barc132



Probe for Locus
BARC132
Locus
Xbarc132
barc132
General Remarks
Contains motif : (CT)20
SSR size is the band size in Chinese Spring
BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type
PCR
SSR
PCR primers
5' GCG GGG GAA AAA CAA GTT GAG ACT TT 3'
5' GCG GTA GTT TGT GGT GAA AAA ATG 3'
SSR size
271
Amplification Conditions
Annealing temperature = 50
Sequence
BV211510
Source Species
Triticum aestivum