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GrainGenes Marker Report: Xbarc133

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Locus	Xbarc133
Type	Microsatellite
Chromosome	3B
Map	Wheat, Avalon x Cadenza 3B 91.7 Wheat, UC1110 x PI610750 3B 6.3 Wheat-Composite2004-3B 16
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Map Data	Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	BARC133
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xbarc133-3B Xbarc133-5D barc133 barc133-3B
Probe for Locus	BARC133
Locus	Xbarc133-3B Xbarc133 Xbarc133-5D barc133 barc133-3B
General Remarks	Contains motif : (CT)24 BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type	PCR SSR
PCR primers	5' AGCGCTCGAAAAGTCAG 3' 5' GGCAGGTCCAACTCCAG 3'
Amplification Conditions	Annealing temperature = 50
Sequence	BV211511
Source Species	Triticum aestivum