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GrainGenes Marker Report: Xbarc199-4B

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Locus
Xbarc199-4B
Type
Microsatellite
Map
Chinese_Spring_Deletion_SSR_4B0.355
[ Show Nearby Loci ]
Breakpoint Interval
C-4BL5-0.71
Map Data
Wheat, Physical, SSR
Probe
BARC199
Possible Orthologs
Xbarc194
Xbarc194-1B
Xbarc199
Xbarc199-2A



Probe for Locus
BARC199
Locus
Xbarc199-2A
Xbarc199
Xbarc199-4B
General Remarks
Contains motif : (ATT)7+(TCA)8
SSR size is the band size in Chinese Spring
BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type
PCR
SSR
PCR primers
5' CGC ATG CTT TCG AAT TTT AAG AAT GTC 3'
5' CGC TGT TGG ATA TTT CTT CTC GGT TTT C 3'
SSR size
258
Amplification Conditions
Annealing temperature = 50
Sequence
BV211540
Source Species
Triticum aestivum