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GrainGenes Marker Report: Xbarc216-5B

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Locus
Xbarc216-5B
Type
Microsatellite
Chromosome
5B
Map
P91193D1/P92201D5-5B42
Ta-Grandin/BR34-5B44.6
Ta-Synthetic/Opata-BARC-5B19
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Map Data
Wheat, Grandin x BR34
Wheat, P91193D1 x P92201D5
Wheat, Synthetic x Opata, BARC
Probe
BARC216
Possible Orthologs
Xbarc216
barc216



Probe for Locus
BARC216
Locus
Xbarc216-5B
Xbarc216
barc216
General Remarks
Contains motif : (CT)22
SSR size is the band size in Chinese Spring
BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type
PCR
SSR
PCR primers
5' TGA CGA CCC AAT CCA TAG ACA 3'
5' GGT GAT TAT TCG TGA GTT CCC TGT G 3'
SSR size
100
Amplification Conditions
Annealing temperature = 52
Sequence
BV211439
Source Species
Triticum aestivum