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GrainGenes Marker Report: Xbarc217

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Locus
Xbarc217
Type
Microsatellite
Chromosome
4D
Map
Wheat, UC1110 x PI610750 4D61.2
Wheat-Composite2004-4D61
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Wheat, UC1110 x PI610750
Probe
BARC217
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs
Xbarc217-4D
barc217



Probe for Locus
BARC217
Locus
Xbarc217-4D
Xbarc217
barc217
General Remarks
Contains motif : (CT)12
SSR size is the band size in Chinese Spring
BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type
PCR
SSR
PCR primers
5' GCG TTG TGT TGA AGG CTG AGC ATC CA 3'
5' GCG GAG TAG CCT AAC GGC GGT GGA GGA AAC 3'
SSR size
95
Amplification Conditions
Annealing temperature = 52
Sequence
BV211649
Source Species
Triticum aestivum