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GrainGenes Marker Report: Xbarc219

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Locus	Xbarc219
Type	Microsatellite
Chromosome	2D
Map	Wheat, UC1110 x PI610750 7A 71.6 Wheat-Composite2004-2D 105
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Map Data	Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	BARC219
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xbarc219-2D Xbarc219-7A barc219 barc219-2D
Probe for Locus	BARC219
Locus	Xbarc219-7A Xbarc219-2D Xbarc219 barc219 barc219-2D
General Remarks	Contains motif : (CT)9+17 SSR size is the band size in Chinese Spring BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type	PCR SSR
PCR primers	5' GCG ATC CCA CAA TGC ATG ACA ACT TC 3' 5' GGA CGT CCG ATC GAA TTG GTT T 3'
SSR size	220
Amplification Conditions	Annealing temperature = 55
Sequence	BV211758
Source Species	Triticum aestivum