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GrainGenes Marker Report: Xbarc221-2B

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Locus
Xbarc221-2B
Type
Microsatellite
Map
Chinese_Spring_Deletion_SSR_2B0.18
[ Show Nearby Loci ]
Breakpoint Interval
C-2BL2-0.36
Map Data
Wheat, Physical, SSR
Probe
BARC221
Possible Orthologs
Xbarc221
Xbarc221-6A
Xbarc221-7A



Probe for Locus
BARC221
Locus
Xbarc221-6A
Xbarc221
Xbarc221-2B
Xbarc221-7A
General Remarks
Contains motif : (CT)22
SSR size is the band size in Chinese Spring
BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type
PCR
SSR
PCR primers
5' GAA GCG ACG AGT GTA GGA CGG TAG C 3'
5' CGG CCA GGA CGG TCA GTT CT 3'
SSR size
210
Amplification Conditions
Annealing temperature = 55
Sequence
BV211369
Source Species
Triticum aestivum