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GrainGenes Marker Report: Xbarc227-4B

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Locus
Xbarc227-4B
Type
Microsatellite
Map
Chinese_Spring_Deletion_SSR_4B0.355
Louise/Penawawa-4B46.1
[ Show Nearby Loci ]
Breakpoint Interval
C-4BL5-0.71
Map Data
Wheat, Louise x Penawawa
Wheat, Physical, SSR
Probe
BARC227
Possible Orthologs
Xbarc227



Probe for Locus
BARC227
Locus
Xbarc227-4B
Xbarc227
General Remarks
Contains motif : (CT)13+6
SSR size is the band size in Chinese Spring
BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type
PCR
SSR
PCR primers
5' GCA CCG AAG CTC TAA TAC CAA ATG T 3'
5' GCG TTG AGG AGT GTT CTC TGT TCT GGA 3'
SSR size
192
Amplification Conditions
Annealing temperature = 58
Sequence
BV211830
Source Species
Triticum aestivum