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GrainGenes Marker Report: Xbarc247

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Locus
Xbarc247
Type
Microsatellite
Map
Wheat, WH542 x Synthetic 5A85.7
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Map Data
Wheat, WH542 x Synthetic
Probe
BARC247



Probe for Locus
BARC247
Locus
Xbarc247
General Remarks
Contains motif : (AAT)13(TTA)12
SSR size is the band size in Chinese Spring
BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type
PCR
SSR
PCR primers
5' GCG TGC AAC TAA ATC TTG TTT TGT AAG 3'
5' GCG GGT ATG TTT GAT TTA ATC ACT GAG 3'
SSR size
250
Amplification Conditions
Annealing temperature = 50
Sequence
BV211550
Source Species
Triticum aestivum