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GrainGenes Marker Report: Xbarc255

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Locus	Xbarc255
Type	Microsatellite
Chromosome	7B
Map	Wheat, UC1110 x PI610750 7B 95.4 Wheat-Composite2004-7B 43
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Map Data	Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	BARC255
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xbarc255-7B barc255 barc255-7B
Probe for Locus	BARC255
Locus	Xbarc255-7B Xbarc255 barc255 barc255-7B
General Remarks	Contains motif : (ATT)16 BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type	PCR SSR
PCR primers	5' GTGGCGGCTTGCGGGTGGCTGAGTA 3' 5' GGGTCGGCTAGCCTTCTTTTATGTT 3'
Amplification Conditions	Annealing temperature = 52
Sequence	BV211333
Source Species	Triticum aestivum