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GrainGenes Marker Report: Xbarc274

Locus
Xbarc274
Type
Microsatellite
Probe
BARC274



Probe for Locus
BARC274
Locus
Xbarc274
General Remarks
Contains motif : (ATT)9
SSR size is the band size in Chinese Spring
BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type
PCR
SSR
PCR primers
5' CAT CGA CGC CCA GGC CCC TCA CAC C 3'
5' TGC TTT GTG CCC CTT TGT GCC ATG T 3'
SSR size
175
Amplification Conditions
Annealing temperature = 57
Sequence
BV211377
Source Species
Triticum aestivum