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GrainGenes Marker Report: Xbarc322-5D

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Locus
Xbarc322-5D
Type
Microsatellite
Chromosome
5D
Map
P91193D1/P92201D5-5D132.6
Ta-Synthetic/Opata-BARC-5D126.7
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Map Data
Wheat, P91193D1 x P92201D5
Wheat, Synthetic x Opata, BARC
Probe
BARC322
Possible Orthologs
Xbarc322
barc322



Probe for Locus
BARC322
Locus
Xbarc322-5D
Xbarc322
barc322
General Remarks
Contains motif : (CT)20
SSR size is the band size in Chinese Spring
BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type
PCR
SSR
PCR primers
5' GAG AAC ATG AAC GTG ATT TAC C 3'
5' CGC AAA CTT GTG TAT CCT TAT C 3'
SSR size
233
Amplification Conditions
Annealing temperature = 52
Sequence
BV211665
Source Species
Triticum aestivum