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GrainGenes Marker Report: Xbarc327

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Locus	Xbarc327
Type	Microsatellite
Chromosome	4A
Map	Wheat, UC1110 x PI610750 4A 128.8 Wheat, WH542 x Synthetic 4A 23.3 Wheat-Composite2004-4A 152
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Map Data	Wheat, Composite, 2004 Wheat, UC1110 x PI610750 Wheat, WH542 x Synthetic
Probe	BARC327
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xbarc327-4A barc327 barc327-4A
Probe for Locus	BARC327
Locus	Xbarc327-4A Xbarc327 barc327 barc327-4A
General Remarks	Contains motif : (TAGA)11(CCAT)4 SSR size is the band size in Chinese Spring BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type	PCR SSR
PCR primers	5' GCG GTA TAG GTA TAT CGA GGC ATA GA 3' 5' GCG AGA GAG CGG TAG AGA TAA ATG 3'
SSR size	248
Amplification Conditions	Annealing temperature = 52
Sequence	BV211667
Source Species	Triticum aestivum