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GrainGenes Marker Report: Xbarc330

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Locus	Xbarc330
Type	Microsatellite
Chromosome	5A
Map	Wheat, UC1110 x PI610750 5A 72.1 Wheat-Composite2004-5A 48
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Map Data	Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	BARC330
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xbarc330-5A barc330 barc330-5A
Probe for Locus	BARC330
Locus	Xbarc330-5A Xbarc330 barc330 barc330-5A
General Remarks	Contains motif : (CT)17 SSR size is the band size in Chinese Spring BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type	PCR SSR
PCR primers	5' GCA CTA AGC GCT CTT TAT TTA C 3' 5' CCT GCA TCT GGT ATG GAG A 3'
SSR size	95
Amplification Conditions	Annealing temperature = 52
Sequence	BV211445
Source Species	Triticum aestivum