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GrainGenes Marker Report: Xbarc332

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Locus	Xbarc332
Type	Microsatellite
Map	Wheat, UC1110 x PI610750 6D 20.8
	[ Show Nearby Loci ]
Map Data	Wheat, UC1110 x PI610750
Probe	BARC332
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	barc332-6D barc332
Probe for Locus	BARC332
Locus	Xbarc332 barc332-6D barc332
General Remarks	Contains motif : (GA)25 SSR size is the band size in Chinese Spring BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type	PCR SSR
PCR primers	5' GCG CAG AAG CCC AGG ACA AGA G 3' 5' GCC TCA CAG TAG CCT CAA GCA GAT 3'
SSR size	175
Amplification Conditions	Annealing temperature = 57
Sequence	BV211807
Source Species	Triticum aestivum