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GrainGenes Marker Report: Xbarc4

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Locus	Xbarc4
Type	Microsatellite
Chromosome	5B
Map	Ta-NxW-5B 46.6 Wheat, UC1110 x PI610750 5B 18 Wheat-Composite2004-5B 45
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	BARC4
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xbarc4-5B barc4 barc4-5B
Probe for Locus	BARC4
Locus	Xbarc4-5B Xbarc4 barc4 barc4-5B
General Remarks	Contains motif : (TTA)15 BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type	PCR SSR
PCR primers	5' GCGTGTTTGTGTCTGCGTTCTA 3' 5' CACCACACATGCCACCTTCTTT 3'
Amplification Conditions	Annealing temperature = 52
Sequence	BV211346
Source Species	Triticum aestivum