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GrainGenes Marker Report: Xbarc54-3A

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Locus
Xbarc54-3A
Type
Microsatellite
Map
Chinese_Spring_Deletion_SSR_3A-0.115
[ Show Nearby Loci ]
Breakpoint Interval
C-3AS2-0.23
Map Data
Wheat, Physical, SSR
Probe
BARC54
Possible Orthologs
Xbarc54-6D
Xbarc54
barc54
barc54-6D



Probe for Locus
BARC54
Locus
Xbarc54-6D
Xbarc54-3A
barc54
Xbarc54
barc54-6D
General Remarks
Contains motif : (TAGA)10
BARC primer pairs are developed by P. Cregan, Q. Song and associates at the USDA-ARS Beltsville Agriculture Research Station. Meiotic map information was developed by J.R. Shi, R. Ward and associates at Michigan State University. Deletion map information was developed by B.Gill, S. Singh and associates at Kansas State University. Funding for this research is provided by USDA-ARS in conjunction with the U.S. Wheat and Barley Scab Initiative.
Type
PCR
SSR
PCR primers
5' GCGAACAGGAGGACAGAGGGCACGAGAG 3'
5' GCGCTTTCCCACGTTCCATGTTTCT 3'
Amplification Conditions
Annealing temperature = 60
Sequence
BV211849
Source Species
Triticum aestivum