GrainGenes Marker Report: Xcfa2028-7A
Locus
Xcfa2028-7A
Type
Microsatellite
Chromosome
7A
Map
Chinese_Spring_Deletion_SSR_7A -0.225 CS/SQ1-7A 76.7 T.b.xT.m.-7A 106.7 Ta-SSR-2004-7A 41.7 Ta-Synthetic/Opata-GPW-7A 97.1
[ Show Nearby Loci ]
Breakpoint Interval
C-7AS8-0.45
Map Data
T. boeoticum x monococcum Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe
CFA2028
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfa2028 cfa2028-7A cfa2028
Probe for Locus
CFA2028
Locus
Xcfa2028-7A Xcfa2028 cfa2028-7A cfa2028
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)21 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TGGGTATGAAAGGCTGAAGG 3'
Reverse 5' ATCGCGACTATTCAACGCTT 3'
SSR size
261
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2028-7A
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