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GrainGenes Marker Report: Xcfa2043a

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Locus	Xcfa2043a
Type	Microsatellite
Chromosome	2A
Map	Wheat-Composite2004-2A 71
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Map Data	Wheat, Composite, 2004
Probe	CFA2043
Possible Orthologs	Xcfa2043-2A Xcfa2043-2B Xcfa2043b cfa2043
Probe for Locus	CFA2043
Locus	Xcfa2043-2A Xcfa2043-2B Xcfa2043a Xcfa2043b cfa2043
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)28 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' CAGCCGAAGAAGGATTTCTG 3' Reverse 5' GAGGCAGGAACTTAGGGGAG 3'
SSR size	202
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	2A/2B
Source Species	Triticum urartu