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GrainGenes Marker Report: Xcfa2070

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Locus	Xcfa2070
Type	Microsatellite
Chromosome	5B
Map	Wheat-Composite2004-5B 30
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Map Data	Wheat, Composite, 2004
Probe	CFA2070
Possible Orthologs	Xcfa2070-5B cfa2070
Probe for Locus	CFA2070
Locus	Xcfa2070-5B Xcfa2070 cfa2070
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)18 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' TCTGAACCCTTGATTTTCCG 3' Reverse 5' TTACTGGCAAGCCAGAACTGT 3'
SSR size	294
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5B
Source Species	Triticum urartu