GrainGenes Marker Report: Xcfa2091-4B
Locus
Xcfa2091-4B
Type
Microsatellite
Map
Chinese_Spring_Deletion_SSR_4B 0.355 Ta-Synthetic/Opata-GPW-4B 27.8
[ Show Nearby Loci ]
Breakpoint Interval
C-4BL5-0.71
Map Data
Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe
CFA2091
Possible Orthologs
Xcfa2091 cfa2091 cfa2091-4B
Probe for Locus
CFA2091
Locus
Xcfa2091-4B Xcfa2091 cfa2091 cfa2091-4B
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CTT)4(TCC)5 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' CCTTCTTAATGACCGGCTCG 3'
Reverse 5' AAACGAAGACGTGGACATGG 3'
SSR size
191
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
4B
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2091-4B
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