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GrainGenes Marker Report: Xcfa2114-6A

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Locus	Xcfa2114-6A
Type	Microsatellite
Map	Louise/Penawawa-6A 27.1 P91193D1/P92201D5-6A 74.3 Ta-Courtot/CS-GPW-6A 171.8
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Map Data	Wheat, Courtot x Chinese Spring, GPW Wheat, Louise x Penawawa Wheat, P91193D1 x P92201D5
Probe	CFA2114
Possible Orthologs	Xcfa2114
Probe for Locus	CFA2114
Locus	Xcfa2114-6A Xcfa2114
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)32 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' ATTGGAAGGCCACGATACAC 3' Reverse 5' CCCGTCGGGTTTTATCTAGC 3'
SSR size	209
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	6A
Source Species	Triticum urartu