GrainGenes Marker Report: Xcfa2121-5A
Locus
Xcfa2121-5A
Type
Microsatellite
Chromosome
5A
Map
Ta-SSR-2004-5A 65.4
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
CFA2121
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xcfa2121-2A
[ Show all 8 ]
Probe for Locus
CFA2121
Locus
Xcfa2121-2A
[ Show all 9 ]
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)20 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TAAATGGCCATCAAGCAATG 3'
Reverse 5' GCTTGTGAACTAATGCCTCCC 3'
SSR size
160
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
4A/2A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2121-5A
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