Query (optional)   in Class  

GrainGenes Marker Report: Xcfa2121b

[Submit comment/correction]

Locus
Xcfa2121b
Type
Microsatellite
Chromosome
2A
Map
Wheat-Composite2004-2A82
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
CFA2121
Possible Orthologs
Xcfa2121-2A
[ Show all 8 ]



Probe for Locus
CFA2121
Locus
Xcfa2121-2A
[ Show all 9 ]
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (CA)20
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' TAAATGGCCATCAAGCAATG 3'
Reverse 5' GCTTGTGAACTAATGCCTCCC 3'
SSR size
160
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
4A/2A
Source Species
Triticum urartu