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GrainGenes Marker Report: Xcfa2134-3B

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Locus	Xcfa2134-3B
Type	Microsatellite
Chromosome	3B
Chromosome Arm	3BL
Map	Kofa/UC1113-3B 116.3 Ta-SSR-2004-3B 75.4 Triticum-AxF-3B 72.7
	[ Show Nearby Loci ]
Map Data	Durum wheat, Kofa x UC1113 Wheat, Arina x Forno Wheat, Consensus SSR, 2004
Species	Triticum aestivum
Probe	CFA2134
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs	cfa2134 Xcfa2134 Xcfa2134-3A cfa2134-3B cfa2134-3A
Probe for Locus	CFA2134
Locus	Xcfa2134-3A [ Show all 6 ]
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TC)n Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TTTACGGGGACAGTATTCGG 3' Reverse 5' AAGACACTCGATGCGGAGAG 3'
SSR size	210
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	3A
Source Species	Triticum urartu