GrainGenes Marker Report: Xcfa2187-5A
Locus
Xcfa2187-5A
Type
Microsatellite
Map
Chinese_Spring_Deletion_SSR_5A -0.865 Ta-Synthetic/Opata-GPW-5A -8.4
[ Show Nearby Loci ]
Breakpoint Interval
5AS3-0.75-0.98
Map Data
Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe
CFA2187
Possible Orthologs
Xcfa2187-1D Xcfa2187c
Probe for Locus
CFA2187
Locus
Xcfa2187-1D Xcfa2187-5A Xcfa2187c
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)17 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TAGCAAAGGGTGCATGTGAG 3'
Reverse 5' GCATGTTACGTCGCTGTTGT 3'
SSR size
151
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
5A/?/1D
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2187-5A
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