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GrainGenes Marker Report: Xcfa2201

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Locus
Xcfa2201
Type
Microsatellite
Chromosome
2A
Map
Wheat, UC1110 x PI610750 2A57.5
Wheat-Composite2004-2A17
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Wheat, UC1110 x PI610750
Probe
CFA2201
Reference
ReferenceLowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs
Xcfa2201-2B
cfa2201-2A
cfa2201
cfa2201-2B



Probe for Locus
CFA2201
Locus
Xcfa2201-2B
Xcfa2201
cfa2201-2A
cfa2201
cfa2201-2B
Reference
ReferenceSourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (CA)14
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' CAAACCAACCTCATTGACCC 3'
Reverse 5' CCACCAGAACTTCAACCTGG 3'
SSR size
216
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2A
Source Species
Triticum urartu