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GrainGenes Marker Report: Xcfa2219

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Locus	Xcfa2219
Type	Microsatellite
Chromosome	1A
Map	Wheat, Finch x Eltan 1A 93.9 Wheat, UC1110 x PI610750 1A 107.6 Wheat-Composite2004-1A 126
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Map Data	T. boeoticum x monococcum Wheat, Composite, 2004 Wheat, Finch x Eltan Wheat, UC1110 x PI610750
Probe	CFA2219
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xcfa2219-1A cfa2219-1A cfa2219
Probe for Locus	CFA2219
Locus	Xcfa2219-1A Xcfa2219 cfa2219-1A cfa2219
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GT)21 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TCTGCCGAGTCACTTCATTG 3' Reverse 5' GACAAGGCCAGTCCAAAAGA 3'
SSR size	223
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1A
Source Species	Triticum urartu