GrainGenes Marker Report: Xcfa2293-7A
Locus
Xcfa2293-7A
Type
Microsatellite
Map
Kofa/UC1113-7A 170.6 Ta-Courtot/CS-GPW-7A 232.4
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Courtot x Chinese Spring, GPW
Probe
CFA2293
Possible Orthologs
Xcfa2293-2A
Probe for Locus
CFA2293
Locus
Xcfa2293-7A Xcfa2293-2A
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)20 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TGGGACTTTGTTGGATTTGTT 3'
Reverse 5' CGCCCTTGTTTGTATTGGTT 3'
SSR size
283
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7A
Source Species
Triticum urartu
GrainGenes Marker Report: Xcfa2293-7A
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