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GrainGenes Marker Report: Xcfd219-5B

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Locus	Xcfd219-5B
Type	Microsatellite
Map	Chinese_Spring_Deletion_SSR_5B 0.42 Ta-Synthetic/Opata-GPW-5B 32.7
	[ Show Nearby Loci ]
Breakpoint Interval	5BL6-0.29-0.55
Map Data	Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe	CFD219
Possible Orthologs	Xcfd219-3D [ Show all 6 ]
Probe for Locus	CFD219
Locus	Xcfd219-3D [ Show all 7 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)13 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GGCCCATCTGTCATTGACTT 3' Reverse 5' CAGCTTGTGTTGCTCGCTTA 3'
SSR size	296
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5B/3D
Source Species	Aegilops tauschii