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GrainGenes Marker Report: Xcfd31

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Locus	Xcfd31
Type	Microsatellite
Chromosome	7D
Map	Ta-NxW-7D 49.8 Wheat-Composite2004-7D 45
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	CFD31
Possible Orthologs	Xcfd31-4A [ Show all 11 ]
Probe for Locus	CFD31
Locus	Xcfd31-7D [ Show all 12 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)51 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GCACCAACCTTGATAGGGAA 3' Reverse 5' GTGCCTGATGATTTTACCCG 3'
SSR size	276
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4A/7D
Source Species	Aegilops tauschii