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GrainGenes Marker Report: Xcfd52-5D

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Locus	Xcfd52-5D
Type	Microsatellite
Map	Chinese_Spring_Deletion_SSR_5D 0.3 Ta-Courtot/CS-GPW-5D 94.4 Ta-Synthetic/Opata-GPW-5D 62.4
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Breakpoint Interval	C-5DL1-0.60
Map Data	Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe	CFD52
Probe for Locus	CFD52
Locus	Xcfd52-5D
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: ? Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' AGACGGTGTTGGTTCCTGAG 3' Reverse 5' TAGTGGCCGGTGGAATTAAA 3'
SSR size	275
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5D
Source Species	Aegilops tauschii