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GrainGenes Marker Report: Xgwm0018-1B

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Locus	Xgwm0018-1B
Type	Microsatellite
Map	P91193D1/P92201D5-1B 152.2
	[ Show Nearby Loci ]
Map Data	Wheat, P91193D1 x P92201D5
Probe	WMS18
Possible Orthologs	Xgwm18 [ Show all 6 ]
Probe for Locus	WMS18
Locus	Xgwm18 [ Show all 7 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023. Roeder MS et al. (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246:327-333.
General Remarks	Insert contains repeat (CA)17GA(TA)4 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. Repeat type (CA)x17GA(TA)x4. Product size 186 bp in Chinese Spring.
Type	PCR SSR
PCR primers	5' TGGCGCCATGATTGCATTATCTTC 3' 5' GGTTGCTGAAGAACCTTATTTAGG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS18
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring