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GrainGenes Marker Report: Xgwm124

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Locus
Xgwm124
Type
Microsatellite
Chromosome
1B
Map
Svevo/Ciccio-1B145.9
Wheat-Composite2004-1B75
[ Show Nearby Loci ]
Breakpoint Interval
1BL2-0.69-0.85
Map Data
Durum wheat, Svevo x Ciccio
Wheat, Composite, 2004
Probe
WMS124
Possible Orthologs
Xgwm124-1B
Xgwm124-1B.1
Xgwm124-1B.2
gwm124
gwm124-1B



Probe for Locus
WMS124
Locus
Xgwm124-1B
[ Show all 6 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)27(GT)18imp
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' GCCATGGCTATCACCCAG 3'
5' ACTGTTCGGTGCAATTTGAG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS124
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring