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GrainGenes Marker Report: Xgwm133

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Locus	Xgwm133
Type	Microsatellite
Chromosome	3A
Chromosome Arm	3AL
Map	Tt-LxG-3A 2.6 Wheat, UC1110 x PI610750 6B 66.1 Wheat_Yr76_2016-3A 53.8
	[ Show Nearby Loci ]
Map Data	T. turgidum, Langdon x G18-16 Wheat, UC1110 x PI610750 Wheat_Yr76_2016
Probe	WMS133
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm133-1B [ Show all 17 ]
Probe for Locus	WMS133
Locus	Xgwm133 [ Show all 18 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)39imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ATCTAAACAAGACGGCGGTG 3' 5' ATCTGTGACAACCGGTGAGA 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS133
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring