Query (optional)   in Class  

GrainGenes Marker Report: Xgwm135-1A.2

[Submit comment/correction]

Locus
Xgwm135-1A.2
Type
Microsatellite
Chromosome
1A
Chromosome Arm
1AS
Map
Triticum-AxF-1A57.7
[ Show Nearby Loci ]
Map Data
Wheat, Arina x Forno
Species
Triticum aestivum
Probe
WMS135
Reference
ReferencePaillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs
Xgwm135
[ Show all 6 ]



Probe for Locus
WMS135
Locus
Xgwm135-1A
[ Show all 7 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)20
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' TGTCAACATCGTTTTGAAAAGG 3'
5' ACACTGTCAACCTGGCAATG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS135
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring