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GrainGenes Marker Report: Xgwm148

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Locus	Xgwm148
Type	Microsatellite
Chromosome	2B
Map	Ta-NxW-2B 96.7 Wheat-Composite2004-2B 62
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMS148
Possible Orthologs	gwm148 Xgwm148-2B gwm148-2B
Probe for Locus	WMS148
Locus	Xgwm148-2B Xgwm148 Ne2 gwm148 gwm148-2B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)22 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GTGAGGCAGCAAGAGAGAAA 3' 5' CAAAGCTTGACTCAGACCAAA 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS148
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring